别名:Keratocan, KTN, Keratan sulfate proteoglycan keratocan, KERA, SLRR2B应用:WB,FCM
反应种属:Human, Mouse
规格:50μl/100μl
Description |
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The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2). |
Specification | |
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Aliases | Keratocan, KTN, Keratan sulfate proteoglycan keratocan, KERA, SLRR2B |
Entrez GeneID | 11081 |
Swissprot | O60938 |
WB Predicted band size | 40.5kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | This KERA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human KERA. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Application | |
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WB | 1/1000 |
FCM | 1/25 |
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Anti-KERA Antibody (C-term) at 1:1000 dilution + Mouse eyeball lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing SK-OV-3 cells stained with P34591(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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