库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to KERA

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别名:Keratocan, KTN, Keratan sulfate proteoglycan keratocan, KERA, SLRR2B
应用:WB,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
The protein encoded by this gene is a keratan sulfate
proteoglycan that is involved in corneal transparency. Defects in
this gene are a cause of autosomal recessive cornea plana 2 (CNA2).
Specification
Aliases Keratocan, KTN, Keratan sulfate proteoglycan keratocan, KERA, SLRR2B
Entrez GeneID 11081
Swissprot O60938
WB Predicted band size 40.5kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This KERA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the C-terminal region of human KERA.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/25

Anti-KERA Antibody (C-term) at 1:1000 dilution + Mouse eyeball lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 41 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing SK-OV-3 cells stained with P34591(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。