Rabbit Polyclonal Antibody to LARS (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Leucine–tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
应用:WB,FCM
反应种属:Mouse
规格：50μl/100μl

Description
This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.

Description

This gene encodes a cytosolic leucine-tRNA synthetase, a
member of the class I aminoacyl-tRNA synthetase family. The encoded
enzyme catalyzes the ATP-dependent ligation of L-leucine to
tRNA(Leu). It is found in the cytoplasm as part of a
multisynthetase complex and interacts with the arginine tRNA
synthetase through its C-terminal domain. Alternatively spliced
transcript variants of this gene have been found; however, their
full-length nature is not known.

Specification
Aliases	Leucine–tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
Entrez GeneID	51520
Swissprot	Q9P2J5
WB Predicted band size	134.5kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Mouse
Immunogen	This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the N-terminal region of human LARS.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
FCM	1/10-1/50

	LARS Antibody (N-term) (Cat. #P30931) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LARS antibody detected the LARS protein (arrow).
	LARS Antibody (N-term) (Cat. #P30931) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.