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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to LARS

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别名:Leucine–tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
LARS, a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. This enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain.
Specification
Aliases Leucine–tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
Entrez GeneID 51520
Swissprot Q9P2J5
WB Predicted band size 134.5kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1118-1145 amino acids from the C-terminal region of human LARS.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000
IHC 1/100-1/500

Anti-LARS Antibody (C-term) at 1:1000 dilution + K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 134 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-LARS Antibody (C-term) (Cat.#P33863) in 293 cell line lysates (35ug/lane). LARS(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with LARS antibody (C-term) (Cat.#P33863), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。