Rabbit Polyclonal Antibody to LAYN

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
Layilin is a recently cloned ∼55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998 ) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin’s ∼50-kDa head domain (amino acids 280–435). Other parts of talin (the ∼220-kDa tail fragment) can bind β-integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact. Layilin’s extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins. Receptor for hyaluronate.

Description

Layilin is a recently cloned ∼55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998 ) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin’s ∼50-kDa head domain (amino acids 280–435). Other parts of talin (the ∼220-kDa tail fragment) can bind β-integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact. Layilin’s extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins. Receptor for hyaluronate.

Specification
Swissprot	Q6UX15
WB Predicted band size	43 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human LAYN
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/50-1/100
ELISA	1/5000-1/10000

	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Jurkat and K562 cell lysates, Primary antibody: P04068(LAYN Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes
	The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P04068(LAYN Antibody) at dilution 1/50. (Original magnification: ×200)