Rabbit Polyclonal Antibody to LEF1

包装: 1G|100G|500G|1KG|100KG

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别名:LEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha
应用:WB,IHC,ICC,FCM,IP
反应种属:Human,Rat
规格：50μl/100μl

Description
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).

Description

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).

Specification
Aliases	LEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha
Entrez GeneID	51176
Swissprot	Q9UJU2
WB Predicted band size	Calculated MW: 44 kDa; Observed MW: 25-58 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat
Immunogen	A synthetic peptide of human LEF1
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of LEF1 in C6 lysates using LEF1 antibody.
	Western blot analysis of LEF1 in Jurkat lysates using LEF1 antibody
	Immunocytochemistry analysis of LEF1 (green) in Jurkat using LEF1 antibody,and DAPI(blue).
	Immunohistochemistry analysis of paraffin-embedded Human tonsil using LEF1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.