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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to LEO1 (N-term)

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别名:RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL
应用:WB
反应种属:Human, Mouse
规格:50μl/100μl

Description
The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. It may be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.
Specification
Aliases RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL
Entrez GeneID 123169
Swissprot Q8WVC0
WB Predicted band size 75.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This LEO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the N-terminal region of human LEO1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000

All lanes : Anti-Leo1 Antibody (N-term) at 1:1000-1:2000 dilution
Lane 1: human skeletal muscle lysate
Lane 2: mouse heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 75 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-Leo1 Antibody (N-term) (Cat. #P34019) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.
Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term) (Cat.#P34019). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。