中文 
别名:Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2应用:WB,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation. |
| Specification | |
|---|---|
| Aliases | Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2 |
| Entrez GeneID | 389421 |
| Swissprot | Q6ZN17 |
| WB Predicted band size | 27.1kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-128 amino acids of human LIN28B. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| ICC | 1/10-1/50 |
| FCM | 1/10-1/50 |
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All lanes : Anti-LIN28B Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lane 3: mouse testis lysate Lane 4: NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Confocal immunofluorescent analysis of LIN28B Antibody (Center) (Cat#P33466) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
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Flow cytometric analysis of HL-60 cells using LIN28B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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