中文 
别名:Lysyl oxidase homolog 4, 143-, Lysyl oxidase-like protein 4, Lysyl oxidase-related protein C, LOXL4, LOXC应用:WB,FCM
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. |
| Specification | |
|---|---|
| Aliases | Lysyl oxidase homolog 4, 143-, Lysyl oxidase-like protein 4, Lysyl oxidase-related protein C, LOXL4, LOXC |
| Entrez GeneID | 84171 |
| Swissprot | Q96JB6 |
| WB Predicted band size | 84.5kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This LOXL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-603 amino acids from the C-terminal region of human LOXL4. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
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All lanes : Anti-LOXL4 Antibody (Center) at 1:2000 dilution Lane 1: Human testis lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing A549 cells stained with P34521(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34521, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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