库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to LYN (N-term)

现货 支持定制

  • 满200元免运费
  • 正品保障 提供发票
  • 现货闪电 当时发货

别名:Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8
应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl

Description
Lyn is a protein tyrosine kinase which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis.
Specification
Aliases Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8
Entrez GeneID 4067
Swissprot P07948
WB Predicted band size 58.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This LYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human LYN.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000-1/2000
IHC 1/100-1/500
FCM 1/10-1/50

Anti-LYN Antibody (E46) at 1:1000 dilution + Raji whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-LYN Antibody (N-term) (Cat.#P33579) in HL60 cell line lysates (35ug/lane). LYN (arrow) was detected using the purified Pab.
LYN Antibody (N-term) (Cat. #P33579) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

暂无评论

发表回复

您的邮箱地址不会被公开。 必填项已用 * 标注


本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。