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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to M Cadherin (CDH15)

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别名:Cadherin-15, Cadherin-14, Muscle cadherin, M-cadherin, CDH15, CDH14, CDH3
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
CDH15 is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. This protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.
Specification
Aliases Cadherin-15, Cadherin-14, Muscle cadherin, M-cadherin, CDH15, CDH14, CDH3
Entrez GeneID 1013
Swissprot P55291
WB Predicted band size 88.9kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This M Cadherin (CDH15) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 739-767 amino acids from the C-terminal region of human M Cadherin (CDH15).
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500

Anti-CDH15 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 89 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of CDH15 (arrow) using rabbit polyclonal CDH15 Antibody (C-term) (Cat.#P33532).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH15 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with CDH15 antibody (C-term) (Cat.#P33532), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。