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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to M4A4A (N-term)

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别名:Membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, Four-span transmembrane protein 1, MS4A4A, 4SPAN1, CD20L1, MS4A4
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
This gene encodes a member of the membrane-spanning 4A
gene family. Members of this nascent protein family are
characterized by common structural features and similar intron/exon
splice boundaries and display unique expression patterns among
hematopoietic cells and nonlymphoid tissues. Alternative splicing
of this gene results in several transcript variants; however, not
all transcripts have been fully described.
Specification
Aliases Membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, Four-span transmembrane protein 1, MS4A4A, 4SPAN1, CD20L1, MS4A4
Entrez GeneID 51338
Swissprot Q96JQ5
WB Predicted band size 25.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This M4A4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-64 amino acids from the N-terminal region of human M4A4A.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/10-1/50

All lanes : Anti-M4A4A Antibody (N-term) at 1:500 dilution
Lane 1: MDA-MB-453 whole cell lysate
Lane 2: WiDr whole cell lysate
Lane 3: Daudi whole cell lysate
Lane 4: Raji whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Observed band size : 17kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

M4A4A Antibody (N-term) (Cat. #P34885) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the M4A4A antibody detected the M4A4A protein (arrow).
M4A4A Antibody (N-term) (Cat. #P34885) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。