Rabbit Polyclonal Antibody to MAP2K3 (Phospho-Thr222)

包装: 1G|100G|500G|1KG|100KG

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别名:MAP2K3; MAPKK 3; MEK3; MP2K3; PRKMK3
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Derijard B., Science 267:682-685(1995)Moriguchi T., J. Biol. Chem. 271:26981-26988(1996)The MGC Project Team; Genome Res. 14:2121-2127(2004).

Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Derijard B., Science 267:682-685(1995)Moriguchi T., J. Biol. Chem. 271:26981-26988(1996)The MGC Project Team; Genome Res. 14:2121-2127(2004).

Specification
Aliases	MAP2K3; MAPKK 3; MEK3; MP2K3; PRKMK3
Entrez GeneID	5606;
Swissprot	P46734
WB Predicted band size	39kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Peptide sequence around phosphorylation site of threonine 222(A-K-T(p)-M-D) derived from Human MAP2K3 .
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/1000
IHC	1/50-1/100

	Western blot analysis of extracts from Jurkat cells treated with serum using MAP2K3 (Phospho-Thr222) Antibody P40444.The lane on the right is treated with the antigen-specific peptide.
	Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody P40444 (left)or the same antibody preincubated with blocking peptide (right).