Rabbit Polyclonal Antibody to MAP3K15 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Mitogen-activated protein kinase kinase kinase 15, Apoptosis signal-regulating kinase 3, MAPK/ERK kinase kinase 15, MEK kinase 15, MEKK 15, MAP3K15, ASK3
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格：50μl/100μl

Description
The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. These family members function in a protein kinase signal transduction cascade, where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K), which then activates a specific MAPK. This MAP3K protein plays an essential role in apoptotic cell death triggered by cellular stresses. [provided by RefSeq].

Description

The protein encoded by this gene is a member of the
mitogen-activated protein kinase (MAPK) family. These family
members function in a protein kinase signal transduction cascade,
where an activated MAPK kinase kinase (MAP3K) phosphorylates and
activates a specific MAPK kinase (MAP2K), which then activates a
specific MAPK. This MAP3K protein plays an essential role in
apoptotic cell death triggered by cellular stresses. [provided by
RefSeq].

Specification
Aliases	Mitogen-activated protein kinase kinase kinase 15, Apoptosis signal-regulating kinase 3, MAPK/ERK kinase kinase 15, MEK kinase 15, MEKK 15, MAP3K15, ASK3
Entrez GeneID	389840
Swissprot	Q6ZN16
WB Predicted band size	147.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This MAP3K15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-342 amino acids from the N-terminal region of human MAP3K15.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Anti-MAP3K15 Antibody (N-term) at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 147 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	MAP3K15 Antibody (N-term) (Cat. #P34094) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAP3K15 antibody detected the MAP3K15 protein (arrow).
	MAP3K15 Antibody (N-term) (Cat. #P34094) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
	MAP3K15 Antibody (N-term) (Cat. #P34094)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAP3K15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.