Rabbit Polyclonal Antibody to MAP3K8 (Ab-400)

包装: 1G|100G|500G|1KG|100KG

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别名:C-COT; cancer Osaka thyroid oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 2.7.11.25; kinase Cot
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.Miyoshi J.,Mol. Cell. Biol. 11:4088-4096(1991).Aoki M., J. Biol. Chem. 268:22723-22732(1993).Chan A.M.,Oncogene 8:1329-1333(1993).

Description

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.Miyoshi J.,Mol. Cell. Biol. 11:4088-4096(1991).Aoki M., J. Biol. Chem. 268:22723-22732(1993).Chan A.M.,Oncogene 8:1329-1333(1993).

Specification
Aliases	C-COT; cancer Osaka thyroid oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 2.7.11.25; kinase Cot
Entrez GeneID	1326;
Swissprot	P41279
WB Predicted band size	60kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human MAP3K8 around the phosphorylation site of serine 400 (C-Q-S(p)-L-D).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using MAP3K8 (Ab-400) antibody P41714.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K8 (Ab-400) antibody P41714.