Rabbit Polyclonal Antibody to MAPK15 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8, MAPK15, ERK7, ERK8
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.

Description

The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.

Specification
Aliases	Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8, MAPK15, ERK7, ERK8
Entrez GeneID	225689
Swissprot	Q8TD08
WB Predicted band size	59.8kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This MAPK15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human MAPK15.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500

	Western blot analysis of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody (N-term) (Cat.#P30195). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).
	Western blot analysis of anti-ERK8 Antibody (N-term) (Cat.#P30195) in T47D cell line lysates (35ug/lane). ERK8 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term)(Cat.#P30195), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.