Rabbit Polyclonal Antibody to MCM3

包装: 1G|100G|500G|1KG|100KG

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别名:HCC5, P1.h, RLFB, P1-MCM3
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Specification
Aliases	HCC5, P1.h, RLFB, P1-MCM3
Swissprot	P25205
WB Predicted band size	91 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Fusion protein of human MCM3
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/50-1/200
ELISA	1/1000-1/5000

	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: K562 cells, Jurkat cells, hela cells, Primary antibody: P07446(MCM3 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
	The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P07446(MCM3 Antibody) at dilution 1/30. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P07446(MCM3 Antibody) at dilution 1/30. (Original magnification: ×200)