库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to MDK

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别名:Midkine, MK, Amphiregulin-associated protein, ARAP, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, MDK, MK1, NEGF2
应用:WB,IHC,ICC
反应种属:Human
规格:50μl/100μl

Description
Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play a role in tumorigenesis, perhaps through its effects on angiogenesis (summary by Reynolds et al., 2004 [PubMed 15197188]).
Specification
Aliases Midkine, MK, Amphiregulin-associated protein, ARAP, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, MDK, MK1, NEGF2
Entrez GeneID 4192
Swissprot P21741
WB Predicted band size 15.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This MDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-143 amino acids from the C-terminal region of human MDK.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000-1/2000
IHC 1/100-1/500
ICC 1/10-1/50

Anti-MDK Antibody (C-term) at 1:2000 dilution + Human pancreas lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 16 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

MDK Antibody (C-term) (Cat. #P34640) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MDK antibody detected the MDK protein (arrow).
Confocal immunofluorescent analysis of MDK Antibody (C-term) (Cat. #P34640) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
MDK Antibody (C-term) (Cat. #P34640)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。