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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to Methionyl tRNA Synthetase

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别名:Methionine–tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2
应用:WB,IHC
反应种属:Human, Mouse
规格:50μl/100μl

Description
Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction:

ATP + L-methionine + tRNAMet AMP + diphosphate + L-methionyl-tRNAMet

The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met).

This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.

Specification
Aliases Methionine–tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2
Entrez GeneID 92935
Swissprot Q96GW9
WB Predicted band size 66.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000
IHC 1/100-1/500

Anti-MARS2 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 67 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-MARS2 Antibody (C-term) (P33872) in mouse testis tissue lysates (35ug/lane). MARS2(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody (C-term) (Cat.#P33872), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。