中文 
别名:Methyltransferase-like protein 4, 211-, METTL4应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | Methyltransferase-like protein 4, 211-, METTL4 |
| Entrez GeneID | 64863 |
| Swissprot | Q8N3J2 |
| WB Predicted band size | 54.0kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This METTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-344 amino acids from the C-terminal region of human METTL4. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/2000 |
| IHC | 1/500 |
| FCM | 1/25 |
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using P34785 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
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All lanes : Anti-METTL4 Antibody (C-term) at 1:2000 dilution Lane 1: Huamn liver lysate Lane 2: Rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing U-2 OS cells stained with P34785(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34785, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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