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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to MFRN2 (N-term)

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别名:Mitoferrin-2, Mitochondrial RNA-splicing protein 3/4 homolog, MRS3/4, hMRS3/4, Mitochondrial iron transporter 2, Solute carrier family 25 member 28, SLC25A28, MFRN2
应用:WB,IHC,FCM
反应种属:Human
规格:50μl/100μl

Description
MFRN2 is mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme.
Specification
Aliases Mitoferrin-2, Mitochondrial RNA-splicing protein 3/4 homolog, MRS3/4, hMRS3/4, Mitochondrial iron transporter 2, Solute carrier family 25 member 28, SLC25A28, MFRN2
Entrez GeneID 81894
Swissprot Q96A46
WB Predicted band size 39.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This MFRN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-64 amino acids from the N-terminal region of human MFRN2.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000-1/2000
IHC 1/100-1/500
FCM 1/10-1/50

Anti-MFRN2 Antibody (N-term) at 1:2000 dilution + MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 39 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of MFRN2 Antibody (N-term) (Cat. #P33511) in Jurkat cell line lysates (35ug/lane).MFRN2 (arrow) was detected using the purified Pab.
MFRN2 Antibody (N-term) (Cat. #P33511) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MFRN2 Antibody (N-term) (Cat. #P33511) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MFRN2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。