Rabbit Polyclonal Antibody to MITF (Ab-180/73)

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别名:MITF M1; Microphthalmia-associated transcription factor;
应用:WB,IHC,ICC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5′-CACGTG-3′) found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.J篓庐n H. Hallsson, Genetics, May 2004; 167: 233 – 241. Eiichi Morii, Blood, Apr 2001; 97: 2038 – 2044. Eiichi Morii, Blood, Sep 2004; 104: 1656 – 1661.

Description

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5′-CACGTG-3′) found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.J篓庐n H. Hallsson, Genetics, May 2004; 167: 233 – 241. Eiichi Morii, Blood, Apr 2001; 97: 2038 – 2044. Eiichi Morii, Blood, Sep 2004; 104: 1656 – 1661.

Specification
Aliases	MITF M1; Microphthalmia-associated transcription factor;
Entrez GeneID	4286;
Swissprot	O75030
WB Predicted band size	52kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthesized non-phosphopeptide derived from human MITF around the phosphorylation site of serine 180/73 (P-N-S(p)-P-M).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100
ICC	1/100-1/500

	Western blot analysis of extracts from HepG2 cells (lane 1) and COLO205 cells (lane 2), using MITF (Ab-180/73) antibody P41648.
	Western blot analysis of extracts from HepG2 cells (Lane 2) and 3T3 cells (Lane 3), using MITF (Ab-180/73) antiobdy P41648. The lane on the left is treated with synthesized peptide.
	Immunofluorescence analysis of HeLa cells, using MITF (Ab-180/73) antibody P41648.
	Immunohistochemistry analysis of paraffin-embedded human skin tissue using MITF (Ab-180/73) antibody P41648.