Rabbit Polyclonal Antibody to mouse BID (S61)

包装: 1G|100G|500G|1KG|100KG

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别名:BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, Bid
应用:WB,IHC,ICC
反应种属:Mouse
规格：50μl/100μl

Description
Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.

Specification
Aliases	BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, Bid
Entrez GeneID	12122
Swissprot	P70444
WB Predicted band size	22.0kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Mouse
Immunogen	This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from mouse BID.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	Western blot analysis of anti-mouse BID Antibody (S61) (Cat.#P30328) in mouse spleen tissue lysates (35ug/lane). BID (arrow) was detected using the purified Pab .
	mouse BID Antibody (S61) (Cat.#P30328) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	mouse BID Antibody (S61)(Cat.#P30328) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry. Clinical relevance has not been evaluated.