别名:Dihydrofolate reductase, Dhfr应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl
Description |
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Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. |
Specification | |
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Aliases | Dihydrofolate reductase, Dhfr |
Entrez GeneID | 13361 |
Swissprot | P00375 |
WB Predicted band size | 21.6kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This Mouse DHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the C-terminal region of mouse DHFR. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Application | |
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WB | 1/2000 |
IHC | 1/100-1/500 |
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All lanes : Anti-DHFR Antibody (C-term) at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: mouse kidney lysate Lane 3: mouse liver lysate Lane 4: mouse thymus lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Anti-DHFR Antibody (C-term) at 1:2000 dilution + PC-12 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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DHFR Antibody (C-term) (Cat.# P34295) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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