库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to Mouse DHFR

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别名:Dihydrofolate reductase, Dhfr
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
Specification
Aliases Dihydrofolate reductase, Dhfr
Entrez GeneID 13361
Swissprot P00375
WB Predicted band size 21.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This Mouse DHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the C-terminal region of mouse DHFR.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/2000
IHC 1/100-1/500

All lanes : Anti-DHFR Antibody (C-term) at 1:2000 dilution
Lane 1: C2C12 whole cell lysate
Lane 2: mouse kidney lysate
Lane 3: mouse liver lysate
Lane 4: mouse thymus lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 22 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-DHFR Antibody (C-term) at 1:2000 dilution + PC-12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 22 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

DHFR Antibody (C-term) (Cat.# P34295) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。