Rabbit Polyclonal Antibody to (Mouse) Epcam

包装: 1G|100G|500G|1KG|100KG

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别名:Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
应用:WB,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

Description

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

Specification
Aliases	Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
Entrez GeneID	17075
Swissprot	Q99JW5
WB Predicted band size	35.0kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This Mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/25

	Western blot analysis of lysates from mouse kidney, mouse lung, mouse testis tissue lysate (from left to right), using Epcam Antibody (C-term)(Cat. #P32922). P32922 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
	Flow cytometric analysis of HepG2 cells using (Mouse) Epcam Antibody (C-term)(green, Cat#P32922) compared to an isotype control of rabbit IgG(blue). P32922 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.