中文 
别名:GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, Eras应用:WB,FCM
反应种属:Mouse
规格：50μl/100μl
| Description |
|---|
| Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells. |
| Specification | |
|---|---|
| Aliases | GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, Eras |
| Entrez GeneID | 353283 |
| Swissprot | Q7TN89 |
| WB Predicted band size | 24.3kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This (Mouse) Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from the N-terminal region of mouse Eras. |
| Application | |
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
 |
Western blot analysis of lysate from mouse F9 cell line, using Eras Antibody (N-term)(Cat. #P32926). P32926 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug. |
 |
Overlay histogram showing F9 cells stained with P32926 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P32926, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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