Rabbit Polyclonal Antibody to Mouse p27Kip1 (C-term T197)

包装: 1G|100G|500G|1KG|100KG

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别名:Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
应用:WB
反应种属:Mouse
规格：50μl/100μl

Description
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E-and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Description

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E-and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Specification
Aliases	Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
Entrez GeneID	12576
Swissprot	P46414
WB Predicted band size	22.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Mouse
Immunogen	This Mouse p27Kip1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-197 amino acids from the C-terminal region of mouse p27Kip1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000

	Anti-Mouse p27Kip1 Antibody (C-term T197) at 1:1000 dilution + C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Mouse p27Kip1 Antibody (C-term T197) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the (mouse) p27Kip1 antibody detected the (mouse) p27Kip1 protein (arrow).