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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to MOX1 (Meox1) (N-term)

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别名:Homeobox protein MOX-1, Mesenchyme homeobox 1, MEOX1, MOX1
应用:WB,ICC
反应种属:Human
规格:50μl/100μl

Description
Meox1 is a member of a subfamily of non-clustered, diverged, antennapedia-like homeobox-containing genes. This protein may play a role in the molecular signaling network regulating somite development.
Specification
Aliases Homeobox protein MOX-1, Mesenchyme homeobox 1, MEOX1, MOX1
Entrez GeneID 4222
Swissprot P50221
WB Predicted band size 28.0kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This MOX1 (Meox1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-38 amino acids from the N-terminal region of human MOX1 (Meox1).
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
ICC 1/10-1/50

Anti-Meox1 (Human N-term) at 1:1000 dilution + human heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 28 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-Meox1 Antibody (N-term) (Cat. #P33577) in 293 cell line lysates (35ug/lane). Meox1(arrow) was detected using the purified Pab.
Fluorescent confocal image of Hela cell stained with Meox1 (Human N-term)(Cat#P33577).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Meox1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Meox1 immunoreactivity is localized to Nucleolus significantly.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。