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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to MPP7 (N-term)

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别名:MAGUK p55 subfamily member 7, MPP7
应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Acts as an important adapter that promotes epithelial cell polarity and tight junction formation via its interaction with DLG1. Involved in the assembly of protein complexes at sites of cell-cell contact.
Specification
Aliases MAGUK p55 subfamily member 7, MPP7
Entrez GeneID 143098
Swissprot Q5T2T1
WB Predicted band size 65.5kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This MPP7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human MPP7.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/500-1/1000
IHC 1/100-1/500
FCM 1/25

All lanes : Anti-MPP7 Antibody (N-term) at 1:1000 dilution
Lane 1: T47D whole cell lysate
Lane 2: Caco2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Observed band size : 66kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-MPP7 Antibody (N-term) at 1:1000 dilution
Lane 1: T47D whole cell lysate
Lane 2: Caco2 whole cell lysate
Lane 3: A431 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(ASP1615) at 1/10000 dilution.

Observed band size : 66kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-MPP7 Antibody (N-term) at 1:1000 dilution
Lane 1: T47D whole cell lysate
Lane 2: Caco2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Observed band size : 66kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of Hela cells using MPP7 Antibody (N-term)(green, Cat#P34968) compared to an isotype control of rabbit IgG(blue). P34968 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. kidney section using MPP7 Antibody (N-term)(Cat#P34968). P34968 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. liver section using MPP7 Antibody (N-term)(Cat#P34968). P34968 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. kidney section using MPP7 Antibody (N-term)(Cat#P34968). P34968 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。