Rabbit Polyclonal Antibody to MRPL32

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别名:39S ribosomal protein L32; mitochondrial [Precursor]; L32mt; MRP-L32; HSPC283
应用:WB,ICC
反应种属:Human
规格：50μl/100μl

Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.Suzuki T., J. Biol. Chem. 276:21724-21736(2001).The MGC Project Team; Genome Res. 14:2121-2127(2004).Kenmochi N., Genomics 77:65-70(2001).

Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.Suzuki T., J. Biol. Chem. 276:21724-21736(2001).The MGC Project Team; Genome Res. 14:2121-2127(2004).Kenmochi N., Genomics 77:65-70(2001).

Specification
Aliases	39S ribosomal protein L32; mitochondrial [Precursor]; L32mt; MRP-L32; HSPC283
Entrez GeneID	64983;
Swissprot	Q9BYC8
WB Predicted band size	21kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized peptide derived from internal of human MRPL32.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
ICC	1/100-1/500

	Western blot analysis of extracts from Jurkat cells, MCF-7 cells and HepG2 cells, using MRPL32 antibody P42229.
	Immunofluorescence analysis of A549 cells, using MRPL32 antibody P42229.