Rabbit Polyclonal Antibody to MRPL54

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别名:39S ribosomal protein 54; mitochondrial; L54mt; mitochondrial ribosomal protein L54; MRP-L54
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.The MGC Project Team; Genome Res. 14:2121-2127(2004).Colinge J., Submitted (OCT-2008) to UniProtKB.

Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.The MGC Project Team; Genome Res. 14:2121-2127(2004).Colinge J., Submitted (OCT-2008) to UniProtKB.

Specification
Aliases	39S ribosomal protein 54; mitochondrial; L54mt; mitochondrial ribosomal protein L54; MRP-L54
Entrez GeneID	116541;
Swissprot	Q6P161
WB Predicted band size	20kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized peptide derived from internal of human MRPL54.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from Jurkat cells, using MRPL54 antibody P42223.
	Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using MRPL54 antibody P42223.