Rabbit Polyclonal Antibody to MRPL9 (N-term)

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别名:39S ribosomal protein L9, mitochondrial, L9mt, MRP-L9, MRPL9
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 8q21.11. [provided by RefSeq].

Description

Mammalian mitochondrial ribosomal proteins are encoded by
nuclear genes and help in protein synthesis within the
mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
small 28S subunit and a large 39S subunit. They have an estimated
75% protein to rRNA composition compared to prokaryotic ribosomes,
where this ratio is reversed. Another difference between mammalian
mitoribosomes and prokaryotic ribosomes is that the latter contain
a 5S rRNA. Among different species, the proteins comprising the
mitoribosome differ greatly in sequence, and sometimes in
biochemical properties, which prevents easy recognition by sequence
homology. This gene encodes a 39S subunit protein. A pseudogene
corresponding to this gene is found at 8q21.11. [provided by
RefSeq].

Specification
Aliases	39S ribosomal protein L9, mitochondrial, L9mt, MRP-L9, MRPL9
Entrez GeneID	65005
Swissprot	Q9BYD2
WB Predicted band size	30.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This MRPL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human MRPL9.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	MRPL9 Antibody (N-term) (Cat. #P31207) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MRPL9 antibody detected the MRPL9 protein (arrow).
	MRPL9 Antibody (N-term) (Cat. #P31207)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MRPL9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.