Rabbit Polyclonal Antibody to MRPS21

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别名:28S ribosomal protein S21; mitochondrial; S21mt; MRP-S21; MRPS21
应用:WB,IHC,ICC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5′ UTR; both transcripts encode the same protein.Suzuki T., J. Biol. Chem. 276:33181-33195(2001).Huang C., Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.The MGC Project Team; Genome Res. 14:2121-2127(2004).

Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5′ UTR; both transcripts encode the same protein.Suzuki T., J. Biol. Chem. 276:33181-33195(2001).Huang C., Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.The MGC Project Team; Genome Res. 14:2121-2127(2004).

Specification
Aliases	28S ribosomal protein S21; mitochondrial; S21mt; MRP-S21; MRPS21
Entrez GeneID	54460;
Swissprot	P82921
WB Predicted band size	11kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from internal of human MRPS21.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100
ICC	1/100-1/500

	Western blot analysis of extracts from COS cells, using MRPS21 antibody P42463.
	Immunofluorescence analysis of MCF-7 cells, using MRPS21 antibody P42463.
	Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using MRPS21 antibody P42463.