Rabbit Polyclonal Antibody to MRPS28

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别名:MRPS35; HSPC007; MRP-S28; MRP-S35
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature.

Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature.

Specification
Aliases	MRPS35; HSPC007; MRP-S28; MRP-S35
Swissprot	Q9Y2Q9
WB Predicted band size	21 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Fusion protein of human MRPS28
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/100-1/300
ELISA	1/5000-1/10000

	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: HL-60, Jurkat and A549 cell lysates, Primary antibody: P03282(MRPS28 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds
	The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P03282(MRPS28 Antibody) at dilution 1/105. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P03282(MRPS28 Antibody) at dilution 1/105. (Original magnification: ×200)