Rabbit Polyclonal Antibody to Mst1 (Phospho-Thr183)

包装: 1G|100G|500G|1KG|100KG

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别名:EC 2.7.11.1; MST-1; Mammalian STE20-like protein kinase 1; STE20-like kinase MST1; STK4; Serine/threonine protein kinase 4; Serine/threonine protein kinase Krs-2; kinase MST1; serine/threonine kinase 4; STE20-like kinase MST1; Yeast Sps1/Ste20-related kin
应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation By similarity.

Description

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation By similarity.

Specification
Aliases	EC 2.7.11.1; MST-1; Mammalian STE20-like protein kinase 1; STE20-like kinase MST1; STK4; Serine/threonine protein kinase 4; Serine/threonine protein kinase Krs-2; kinase MST1; serine/threonine kinase 4; STE20-like kinase MST1; Yeast Sps1/Ste20-related kin
Entrez GeneID	6789;
Swissprot	Q13043
WB Predicted band size	60kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Peptide sequence around phosphorylation site of threonine 183 (R-N-T(p)-V-I) derived from Human Mst1.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Mst1 (Phospho-Thr183) antibody P40749. The lane on the right is treated with the synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Mst1 (Phospho-Thr183) antibody P40749. The picture on the right is treated with the synthesized peptide.