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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to MSX1

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别名:Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein, MSX1, HOX7
应用:WB,ICC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
This gene encodes a member of the muscle segment homeobox
gene family. The encoded protein functions as a transcriptional
repressor during embryogenesis through interactions with components
of the core transcription complex and other homeoproteins. It may
also have roles in limb-pattern formation, craniofacial
development, particularly odontogenesis, and tumor growth
inhibition. Mutations in this gene, which was once known as
homeobox 7, have been associated with nonsyndromic cleft lip with
or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome,
and autosomoal dominant hypodontia.
Specification
Aliases Homeobox protein MSX-1, Homeobox protein Hox-7, Msh homeobox 1-like protein, MSX1, HOX7
Entrez GeneID 4487
Swissprot P28360
WB Predicted band size 31.5kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This MSX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human MSX1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000-1/2000
ICC 1/10-1/50

All lanes : Anti-MSX1 Antibody (Center) at 1:1000 dilution
Lane 1: HepG2 MG whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: A375 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Observed band size : 32kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

MSX1 Antibody (Center) (Cat. #P34893) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).
Fluorescent image of U251 cell stained with MSX1 Antibody (Center)(Cat#P34893).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MSX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).MSX1 immunoreactivity is localized to Nucleus significantly.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。