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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to MVD

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别名:Diphosphomevalonate decarboxylase, Mevalonate (diphospho)decarboxylase, MDDase, Mevalonate pyrophosphate decarboxylase, MVD, MPD
应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
The enzyme mevalonate pyrophosphate decarboxylase catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP.
Specification
Aliases Diphosphomevalonate decarboxylase, Mevalonate (diphospho)decarboxylase, MDDase, Mevalonate pyrophosphate decarboxylase, MVD, MPD
Entrez GeneID 4597
Swissprot P53602
WB Predicted band size 43.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This MVD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the Central region of human MVD.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

All lanes : Anti-MVD Antibody (Center) at 1:1000 dilution
Lane 1: HL-60 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.

Observed band size : 43kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of MVD Antibody (Center)(Cat. #P34941) in HL60 cell line lysates (35ug/lane). MVD (arrow) was detected using the purified Pab.
MVD Antibody (Center) (Cat. #P34941) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MVD Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。