Rabbit Polyclonal Antibody to NGEF

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别名:EPHEXIN; ARHGEF27
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

Description

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

Specification
Aliases	EPHEXIN; ARHGEF27
Swissprot	Q8N5V2
WB Predicted band size	82 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human NGEF
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/30-1/150
ELISA	1/5000-1/10000

	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Rat brain tissue and Mouse brain tissue lysates, Primary antibody: P02940(NGEF Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
	The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P02940(NGEF Antibody) at dilution 1/35. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P02940(NGEF Antibody) at dilution 1/35. (Original magnification: ×200)