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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to NKX2-1 (N-term)

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别名:Homeobox protein Nkx-21, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1, Thyroid-specific enhancer-binding protein, T/EBP, NKX2-1, NKX2A, TITF1, TTF1
应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
This gene encodes a protein initially identified as a
thyroid-specific transcription factor. The encoded protein binds to
thyroglobulin promoter and regulates the expression of
thyroid-specific genes but has also been shown to regulate the
expression of genes involved in morphogenesis. Mutations and
deletions in this gene are associated with benign hereditary
chorea, choreoathetosis, congenital hypothyroidism, and neonatal
respiratory distress, and may be associated with thyroid cancer.
Multiple transcript variants encoding different isoforms have been
found for this gene.
Specification
Aliases Homeobox protein Nkx-21, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1, Thyroid-specific enhancer-binding protein, T/EBP, NKX2-1, NKX2A, TITF1, TTF1
Entrez GeneID 7080
Swissprot P43699
WB Predicted band size 38.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This NKX2-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NKX2-1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
FCM 1/10-1/50

Anti-NKX2-1 Antibody (N-term) at 1:1000 dilution + TT cell nucleus lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 39 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

NKX2-1 Antibody (N-term) (Cat. #P33594) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NKX2-1 antibody detected the NKX2-1 protein (arrow).
NKX2-1 Antibody (N-term) (Cat. #P33594) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。