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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to NNMT

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别名:Nicotinamide N-methyltransferase, NNMT
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is the protein responsible for this enzymatic activity, which uses S-adenosyl methionine as the methyl donor.
Specification
Aliases Nicotinamide N-methyltransferase, NNMT
Entrez GeneID 4837
Swissprot P40261
WB Predicted band size 29.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NNMT.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000-1/2000
IHC 1/100-1/500

All lanes : Anti-NNMT Antibody (Center) at 1:2000 dilution
Lane 1: A549 whole cell lysate
Lane 2: human liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 30 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-NNMT Antibody (Center) at 1:2000 dilution
Lane 1: A549 whole cell lysate
Lane 2: human liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 30 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of NNMT (arrow) using rabbit polyclonal NNMT Antibody (Center)(Cat# P33590). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT antibody (Center) (Cat# P33590), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。