别名:Nicotinamide N-methyltransferase, NNMT应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl
Description |
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N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is the protein responsible for this enzymatic activity, which uses S-adenosyl methionine as the methyl donor. |
Specification | |
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Aliases | Nicotinamide N-methyltransferase, NNMT |
Entrez GeneID | 4837 |
Swissprot | P40261 |
WB Predicted band size | 29.6kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NNMT. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Application | |
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WB | 1/1000-1/2000 |
IHC | 1/100-1/500 |
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All lanes : Anti-NNMT Antibody (Center) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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All lanes : Anti-NNMT Antibody (Center) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of NNMT (arrow) using rabbit polyclonal NNMT Antibody (Center)(Cat# P33590). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NNMT gene (Lane 2) (Origene Technologies). |
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with NNMT antibody (Center) (Cat# P33590), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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