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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to NOX1

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别名:NADPH oxidase 1, NOX-1, 1—, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, NOX1, MOX1, NOH1
应用:WB,IHC
反应种属:Human
规格:50μl/100μl

Description
Voltage-gated proton (hydrogen) channels play an important
role in cellular defense against acidic stress. They are unique
among ion channels with respect to their extremely high
selectivity, marked temperature dependence, and unitary
conductance, which is 3 orders of magnitude lower than that of most
other ion channels. NOX1 is a homolog of the catalytic subunit of
the superoxide-generating NADPH oxidase of phagocytes, gp91phox.
Two transcript variants encoding different isoforms have been found
for this gene.
Specification
Aliases NADPH oxidase 1, NOX-1, 1—, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, NOX1, MOX1, NOH1
Entrez GeneID 27035
Swissprot Q9Y5S8
WB Predicted band size 64.9kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This NOX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the Central region of human NOX1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/500

All lanes : Anti-NOX1 Antibody (Center) at 1:1000 dilution
Lane 1: WiDr whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: human uterus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 65 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human colon tissue using P34662 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using P34662 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。