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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to NRAS

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别名:GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Specification
Aliases GTPase NRas, Transforming protein N-Ras, NRAS, HRAS1
Entrez GeneID 4893
Swissprot P01111
WB Predicted band size 21.2kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This NRAS antibody is generated from a rabbit immunized with arecombinant protein from the human region of human NRAS.
Application
WB 1/2000
IHC 1/100-1/500

Anti-NRAS Antibody at 1:2000 dilution + PC-12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 21 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P34483 staining NRAS in human small intestine tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。