中文 
别名:NSFL1 cofactor p47, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1C, UBXN2C应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). |
| Specification | |
|---|---|
| Aliases | NSFL1 cofactor p47, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1C, UBXN2C |
| Entrez GeneID | 55968 |
| Swissprot | Q9UNZ2 |
| WB Predicted band size | 40.6kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NSFL1C antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 214-248 amino acids from the Central region of human NSFL1C. |
| Application | |
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/25 |
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All lanes : Anti-NSFL1C Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lane 4: U-251 MG whole cell lysate Lane 5: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing A431 cells stained with P34417(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
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P34417 staining NSFL1C in human testis tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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