中文 
别名:NT5C3; P5N1; UMPH1; Cytosolic 5′-nucleotidase 3A; Cytosolic 5′-nucleotidase 3; Cytosolic 5′-nucleotidase III; cN-III; Pyrimidine 5′-nucleotidase 1; P5’N-1; P5N-1; PN-I; Uridine 5′-monophosphate hydrolase 1; p36应用:WB,IHC,ICC
反应种属:Human,Mouse
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | NT5C3; P5N1; UMPH1; Cytosolic 5′-nucleotidase 3A; Cytosolic 5′-nucleotidase 3; Cytosolic 5′-nucleotidase III; cN-III; Pyrimidine 5′-nucleotidase 1; P5’N-1; P5N-1; PN-I; Uridine 5′-monophosphate hydrolase 1; p36 |
| Entrez GeneID | 51251; |
| Swissprot | Q9H0P0 |
| WB Predicted band size | 38kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NT5C3. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Application | |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| ICC | 1/100-1/300 |
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Western blot analysis of MRPS7 expression in rat tongue whole cell lysates. |
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Immunofluorescent analysis of NT5C3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
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Immunohistochemical analysis of NT5C3 staining in human brain formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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