Rabbit Polyclonal Antibody to NUP153 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Nuclear pore complex protein Nup153, 153 kDa nucleoporin, Nucleoporin Nup153, NUP153
应用:WB,FCM
反应种属:Human
规格：50μl/100μl

Description
Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. This protein has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.

Description

Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. This protein has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.

Specification
Aliases	Nuclear pore complex protein Nup153, 153 kDa nucleoporin, Nucleoporin Nup153, NUP153
Entrez GeneID	9972
Swissprot	P49790
WB Predicted band size	153.9kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This NUP153 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of human NUP153.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/1000
FCM	1/10-1/50

	Western blot analysis of NUP153 Antibody (N-term) (Cat. #P30457) in Hela cell line lysates (35ug/lane). NUP153 (arrow) was detected using the purified Pab.
	NUP153 Antibody (N-term)(Cat. #P30457) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.