Rabbit Polyclonal Antibody to p14 ARF

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:CDK4I; CMM2; MLM; MTS1; TP16
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its ‘Lys63’-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform isstabilized by C1QBP. UniProtKB Q64364B篓娄atrice Eymin, Mol. Cell. Biol., Jun 2006; 26: 4339 – 4350. Guillaume Normand, J. Biol. Chem., Feb 2005; 280: 7118 – 7130. Abel S篓垄nchez-Aguilera, Blood, Feb 2002; 99: 1411 – 1418. Manel Esteller, Cancer Res., Apr 2001; 61: 2816 – 2821.

Description

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its ‘Lys63’-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform isstabilized by C1QBP. UniProtKB Q64364B篓娄atrice Eymin, Mol. Cell. Biol., Jun 2006; 26: 4339 – 4350. Guillaume Normand, J. Biol. Chem., Feb 2005; 280: 7118 – 7130. Abel S篓垄nchez-Aguilera, Blood, Feb 2002; 99: 1411 – 1418. Manel Esteller, Cancer Res., Apr 2001; 61: 2816 – 2821.

Specification
Aliases	CDK4I; CMM2; MLM; MTS1; TP16
Entrez GeneID	1029;
Swissprot	P42771
WB Predicted band size	18kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized peptide derived from human p14 ARF antibody.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100
ICC	1/100-1/500

	Western blot analysis of extracts from HeLa cells, using p14 ARF antibody P41826.
	Western blot analysis of extracts from 293 cells (Lane 2), using p14 ARF antiobdy P41826. The lane on the left is treated with synthesized peptide.
	Immunofluorescence analysis of HeLa cells, using p14 ARF antibody P41826.
	Immunohistochemical analysis of paraffin-embedded human placenta tissue using p14 ARF antibody P41826.