Rabbit Polyclonal Antibody to PAFAH1B1 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Platelet-activating factor acetylhydrolase IB subunit alpha {ECO:0000255|HAMAP-Rule:MF_03141}, Lissencephaly-1 protein {ECO:0000255|HAMAP-Rule:MF_03141}, LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141}, PAF acetylhydrolase 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}, PAF-AH 45 kDa subunit {ECO:0000255|HAMAP-Rule:MF_03141}, PAF-AH alpha {ECO:0000255|HAMAP-Rule:MF_03141}, PAFAH alpha {ECO:0000255|HAMAP-Rule:MF_03141}, PAFAH1B1 {ECO:0000255|HAMAP-Rule:MF_03141}
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.

Description

Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.

Specification
Aliases	Platelet-activating factor acetylhydrolase IB subunit alpha {ECO:0000255\|HAMAP-Rule:MF_03141}, Lissencephaly-1 protein {ECO:0000255\|HAMAP-Rule:MF_03141}, LIS-1 {ECO:0000255\|HAMAP-Rule:MF_03141}, PAF acetylhydrolase 45 kDa subunit {ECO:0000255\|HAMAP-Rule:MF_03141}, PAF-AH 45 kDa subunit {ECO:0000255\|HAMAP-Rule:MF_03141}, PAF-AH alpha {ECO:0000255\|HAMAP-Rule:MF_03141}, PAFAH alpha {ECO:0000255\|HAMAP-Rule:MF_03141}, PAFAH1B1 {ECO:0000255\|HAMAP-Rule:MF_03141}
Entrez GeneID	5048
Swissprot	P43034
WB Predicted band size	46.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This PAFAH1B1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 76-110 amino acids from the N-terminal region of human PAFAH1B1.

Application
WB	1/1000
IHC	1/100-1/500

	Western blot analysis of lysates from HeLa, mouse C2C12, rat C6 cell line (from left to right), using PAFAH1B1 Antibody (N-term)(Cat. #P32808). P32808 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
	Immunohistochemical analysis of paraffin-embedded H. brain section using PAFAH1B1 Antibody (N-term)(Cat#P32808). P32808 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.