Rabbit Polyclonal Antibody to PAK1/2/3 (Ab-423/402/421)

包装: 1G|100G|500G|1KG|100KG

现货支持定制

QQ咨询
| 微信咨询

400-880-2824

满200元免运费
正品保障提供发票
现货闪电当时发货

别名:EC 2.7.11.1; Gamma-PAK; P21-activated kinase 2; PAK 2; PAK-2
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.Bernadett Boda, J. Neurosci., Dec 2004; 24: 10816 – 10825. Donna L. McPhie, J. Neurosci., Jul 2003; 23: 6914. Jin-Hun Jung, J. Biol. Chem., Dec 2005; 280: 40025 – 40031. Guo-Lei Zhou, Mol. Cell. Biol., Nov 2003; 23: 8058 – 8069.

Description

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.Bernadett Boda, J. Neurosci., Dec 2004; 24: 10816 – 10825. Donna L. McPhie, J. Neurosci., Jul 2003; 23: 6914. Jin-Hun Jung, J. Biol. Chem., Dec 2005; 280: 40025 – 40031. Guo-Lei Zhou, Mol. Cell. Biol., Nov 2003; 23: 8058 – 8069.

Specification
Aliases	EC 2.7.11.1; Gamma-PAK; P21-activated kinase 2; PAK 2; PAK-2
Entrez GeneID	5058;
Swissprot	Q13153
WB Predicted band size	62kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human PAK1/2/3 around the phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from NIH-3T3 cells, using PAK1/2/3 (Ab-423/402/421) antibody P41650.
	Western blot analysis of extracts from 293 cells (Lane 2), using PAK1/2/3 (Ab-423/402/421) antiobdy P41650. The lane on the left is treated with synthesized peptide.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using PAK1/2/3 (Ab-423/402/421) antiobdy P41650.