Rabbit Polyclonal Antibody to PAK3 (Ab-154)

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别名:beta-PAK; CDC42/RAC effector kinase PAK-B; EC 2.7.1.; EC 2.7.11.1; kinase PAK3
应用:WB
反应种属:Human,Rat
规格：50μl/100μl

Description
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.Allen K.M., Nat. Genet. 20:25-30(1998).Jiang C., Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.Kitano T., Mol. Biol. Evol. 20:1281-1289(2003).

Description

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.Allen K.M., Nat. Genet. 20:25-30(1998).Jiang C., Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.Kitano T., Mol. Biol. Evol. 20:1281-1289(2003).

Specification
Aliases	beta-PAK; CDC42/RAC effector kinase PAK-B; EC 2.7.1.; EC 2.7.11.1; kinase PAK3
Entrez GeneID	5063;
Swissprot	O75914
WB Predicted band size	72kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat
Immunogen	Synthesized non-phosphopeptide derived from human PAK3 around the phosphorylation site of serine 154 (Y-M-S(p)-F-T).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000

	Western blot analysis of extracts from rat heart, using PAK3 (Ab-154) antibody P41723.
	Western blot analysis of extracts from HeLa cells (Lane 2), using PAK3 (Ab-154) antiobdy P41723. The lane on the left is treated with systhesized peptide.