Rabbit Polyclonal Antibody to PAK6

包装: 1G|100G|500G|1KG|100KG

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别名:Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6, PAK-6, PAK6, PAK5
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Description

This gene encodes a member of the p21-activated kinase
(PAK) family. The proteins of this family are Rac/Cdc42-associated
Ste20-like Ser/Thr protein kinases, characterized by a highly
conserved amino-terminal Cdc42/Rac interactive binding (CRIB)
domain and a carboxyl-terminal kinase domain. PAK kinases are
implicated in the regulation of a number of cellular processes,
including cytoskeleton rearrangement, apoptosis and the MAP kinase
signaling pathway. The protein encoded by this gene was found to
interact with androgen receptor (AR), which is a steroid
hormone-dependent transcription factor that is important for male
sexual differentiation and development. This gene was found to be
highly expressed in testis and prostate tissues and the encoded
protein was shown to cotranslocate into the nucleus with AR in
response to androgen. Alternatively spliced transcript variants
encoding the same protein have been found for this gene. [provided
by RefSeq].

Specification
Aliases	Serine/threonine-protein kinase PAK 6, PAK-5, p21-activated kinase 6, PAK-6, PAK6, PAK5
Entrez GeneID	106821730;56924
Swissprot	Q9NQU5
WB Predicted band size	74.9kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This PAK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 268-297 amino acids from the Central region of human PAK6.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	All lanes : Anti-PAK6 Antibody (Center) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	PAK6 Antibody (Center) (Cat. #P33603) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PAK6 antibody detected the PAK6 protein (arrow).
	Confocal immunofluorescent analysis of PAK6 Antibody (Center)(Cat#P33603) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	PAK6 Antibody (Center) (Cat. #P33603)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAK6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.