Rabbit Polyclonal Antibody to Parkin

包装: 1G|100G|500G|1KG|100KG

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别名:E3 ubiquitin-protein ligase parkin, 632-, Parkinson juvenile disease protein 2, Parkinson disease protein 2, PARK2, PRKN
应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known; however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function.

Description

Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known; however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function.

Specification
Aliases	E3 ubiquitin-protein ligase parkin, 632-, Parkinson juvenile disease protein 2, Parkinson disease protein 2, PARK2, PRKN
Entrez GeneID	5071
Swissprot	O60260
WB Predicted band size	51.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-417 amino acids from the C-terminal region of human Parkin.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Application
WB	1/500-1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	All lanes : Anti-Parkin Antibody (C-term) at 1:500 dilution + Hela cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Park2 Antibody (C-term) (Cat. #P35080) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Park2 antibody detected the Park2 protein (arrow).
	The anti-Parkin (C-term) Pab (Cat. #P35080) is used in Western blot to detect Parkin in mouse kidney tissue lysate.
	Confocal immunofluorescent analysis of Parkin Antibody (C-term)(Cat#P35080) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	Parkin Antibody (C-term) (Cat. #P35080) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) (Cat.#P35080), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.